Difference between revisions of "Part:BBa K1442102:Design"
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<p>The GFP has been codon optimised for E.coli IDTs codon optimising software.</p> | <p>The GFP has been codon optimised for E.coli IDTs codon optimising software.</p> | ||
<p>The GFP and RBS are in the system in the reverse complement as they are transcribed to forwards sense in the incidence of RdRp functioning correctly.</p> | <p>The GFP and RBS are in the system in the reverse complement as they are transcribed to forwards sense in the incidence of RdRp functioning correctly.</p> | ||
| + | <p>Two stop codons are added after the T7 promoter to ensure no translation occurs here.</p> | ||
===Source=== | ===Source=== | ||
Latest revision as of 22:03, 15 October 2014
Reverse GFP for E.coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 742
Illegal NgoMIV site found at 770
Illegal NgoMIV site found at 799 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 763
Design Notes
There are two restriction sites surrounding the 3’UTR AflII and BsiW1 which can be used to change the RDRP promotor.
The GFP has been codon optimised for E.coli IDTs codon optimising software.
The GFP and RBS are in the system in the reverse complement as they are transcribed to forwards sense in the incidence of RdRp functioning correctly.
Two stop codons are added after the T7 promoter to ensure no translation occurs here.
Source
The sequence for GFP was obtained from the NCBI database and was codon optimised.