Difference between revisions of "Part:BBa K1475003:Design"
 
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===Design Notes===
 
===Design Notes===
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The part was constructed by PCR from the iGEM registry part TetR+LVA ([https://parts.igem.org/Part:BBa_K1475003 Part:BBa_C0040]) using the following primers:
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The part was constructed by PCR from the iGEM registry part TetR+LVA ([https://parts.igem.org/Part:BBa_C0040 Part:BBa_C0040]) using the following primers, which are designed to remove the LVA rapid [https://parts.igem.org/Protein_domains/Degradation degradation tag]:
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Forward primer:  
+
<br>
 +
Forward primer (containing prefix):  
 
<br>
 
<br>
 
AAGAATTCGCGGCCGCTTCTAGATGTCCAGATTAGATAAAAGTAAAGTGATTAACAGCG
 
AAGAATTCGCGGCCGCTTCTAGATGTCCAGATTAGATAAAAGTAAAGTGATTAACAGCG
 
<br>
 
<br>
−
Reverse primer:
+
Reverse primer (containing suffix):
 
<br>
 
<br>
 
TTTCTGCAGCGGCCGCTACTAGTATTATTAGGACCCACTTTCACATTTAAGTTGTTTTTC
 
TTTCTGCAGCGGCCGCTACTAGTATTATTAGGACCCACTTTCACATTTAAGTTGTTTTTC
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−
 
−
 
  
 
===Source===
 
===Source===
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Organizm: ''Escherichia coli''
 +
  
−
This part was constructed from the tetracycline repressor from transposon Tn10 (+LVA) (Part:BBa_C0040) using primers to remove the LVA protein digestion tag. That is THIS PART DOES NOT CONTAIN THE LVA PROTEIN DIGESTION TAG.
 
  
 
===References===
 
===References===
 +
1. C. Krafft, et al.: Interaction of Tet Repressor with Operator DNA and with Tetracycline Studied by Infrared and Raman Spectroscopy. Biophysical Journal, Volume 74, Issue 1, January 1998, Pages 63–71. http://www.sciencedirect.com/science/article/pii/S0006349598777677
 +
<br>
 +
2. Tetsystems, 2008: Principles and Components Description. http://www.tetsystems.com/science-technology/principles-components

Latest revision as of 12:16, 16 October 2014

tetracyclin repressor from transposon Tn10


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was constructed by PCR from the iGEM registry part TetR+LVA (Part:BBa_C0040) using the following primers, which are designed to remove the LVA rapid degradation tag:
Forward primer (containing prefix):
AAGAATTCGCGGCCGCTTCTAGATGTCCAGATTAGATAAAAGTAAAGTGATTAACAGCG
Reverse primer (containing suffix):
TTTCTGCAGCGGCCGCTACTAGTATTATTAGGACCCACTTTCACATTTAAGTTGTTTTTC

Source

Organizm: Escherichia coli


References

1. C. Krafft, et al.: Interaction of Tet Repressor with Operator DNA and with Tetracycline Studied by Infrared and Raman Spectroscopy. Biophysical Journal, Volume 74, Issue 1, January 1998, Pages 63–71. http://www.sciencedirect.com/science/article/pii/S0006349598777677
2. Tetsystems, 2008: Principles and Components Description. http://www.tetsystems.com/science-technology/principles-components