Difference between revisions of "Part:BBa K1554004:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The assembly was performed by GoldenBraid 2.0, a Golden-Gate based assembly system. The barnase was amplified by PCR from [https://parts.igem.org/Part:BBa_J72173 Part:BBa_J72173] and a mutation in T105P was introduced. The used to perform the assembly was [https://parts.igem.org/Part:BBa_K1554006 Part:BBa_K1554006], a vector with the BioBricks backbone pSB1A3 and compatible with the Golden Gate-based assembly system GoldenBraid 2.0. | + | The assembly was performed by GoldenBraid 2.0, a Golden-Gate based assembly system. The barnase was amplified by PCR from [https://parts.igem.org/Part:BBa_J72173 Part:BBa_J72173] and a mutation in T105P was introduced. The vector used to perform the assembly was [https://parts.igem.org/Part:BBa_K1554006 Part:BBa_K1554006], a vector with the BioBricks backbone pSB1A3 and compatible with the Golden Gate-based assembly system GoldenBraid 2.0. |
===Source=== | ===Source=== | ||
| − | TA29 promoter [https://parts.igem.org/Part:BBa_K1554000 Part:BBa_K1554000] | + | TA29 promoter: [https://parts.igem.org/Part:BBa_K1554000 Part:BBa_K1554000] |
| − | Barnase [https://parts.igem.org/Part:BBa_J72173 Part:BBa_J72173] | + | Barnase: [https://parts.igem.org/Part:BBa_J72173 Part:BBa_J72173] |
| − | AmilGFP [https://parts.igem.org/Part:BBa_J72173 Part:BBa_K1467201] | + | AmilGFP: [https://parts.igem.org/Part:BBa_J72173 Part:BBa_K1467201] |
| − | P35s [https://parts.igem.org/Part:BBa_K1537015 Part:BBa_K1537015] | + | P35s: [https://parts.igem.org/Part:BBa_K1537015 Part:BBa_K1537015] |
T35s: GoldenBraid 2.0 Collection | T35s: GoldenBraid 2.0 Collection | ||
Latest revision as of 20:17, 15 October 2014
Yellow biosafety module for plants
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The assembly was performed by GoldenBraid 2.0, a Golden-Gate based assembly system. The barnase was amplified by PCR from Part:BBa_J72173 and a mutation in T105P was introduced. The vector used to perform the assembly was Part:BBa_K1554006, a vector with the BioBricks backbone pSB1A3 and compatible with the Golden Gate-based assembly system GoldenBraid 2.0.
Source
TA29 promoter: Part:BBa_K1554000
Barnase: Part:BBa_J72173
AmilGFP: Part:BBa_K1467201
P35s: Part:BBa_K1537015
T35s: GoldenBraid 2.0 Collection
References
References
Mariani C, Beuckeleer MD, Truettner J, Leemans J, Goldberg RB (1990) Induction of male sterility in plants by a chimaeric ribonuclease gene. Nature 347: 737-741.
Cho HJ, Kim S, Kim M, Kim BD (2001) Production of transgenic male sterile tobacco plants with the cDNA encoding a ribosome inactivating protein in Dianthus sinensis L. Mol Cells 11: 326-333.
Sa G, Mi M, He-Chun Y, Guo-Feng L (2002) Anther-specific expression of ipt gene in transgenic tobacco and its effect on plant development. Transgenic Res 11: 269-278.
Shaya F, Gaiduk S, Keren I, Shevtsov S, Zemah H, et al. (2012) Expression of mitochondrial gene fragments within the tapetum induce male sterility by limiting the biogenesis of the respiratory machinery in transgenic tobacco. J Integr Plant Biol 54: 115-130.
Kriete G, Niehaus K, Perlick AM, Puhler A, Broer I (1996) Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin. Plant J 9: 809-818.
Shukla P, Singh NK, Kumar D, Vijayan S, Ahmed I, et al. (2014) Expression of a pathogen-induced cysteine protease (AdCP) in tapetum results in male sterility in transgenic tobacco. Funct Integr Genomics 14: 307-317.
Goldman MH, Goldberg RB, Mariani C (1994) Female sterile tobacco plants are produced by stigma-specific cell ablation. EMBO J 13: 2976-2984.
Wang HZ, Hu B, Chen GP, Shi NN, Zhao Y, et al. (2008) Application of Arabidopsis AGAMOUS second intron for the engineered ablation of flower development in transgenic tobacco. Plant Cell Rep 27: 251-259.
Sarrion-Perdigones A, Falconi EE, Zandalinas SI, Juarez P, Fernandez-del-Carmen A, et al. (2011) GoldenBraid: an iterative cloning system for standardized assembly of reusable genetic modules. PLoS One 6: e21622.