Difference between revisions of "Part:BBa K1475010:Design"
 
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===Design Notes===
 
===Design Notes===
 
The part was constructed using standard assembly from: [https://parts.igem.org/Part:BBa_K1475003 Part: BBa_K1475003] and [https://parts.igem.org/Part:BBa_K118024 Part:BBa_K118024]
 
The part was constructed using standard assembly from: [https://parts.igem.org/Part:BBa_K1475003 Part: BBa_K1475003] and [https://parts.igem.org/Part:BBa_K118024 Part:BBa_K118024]
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===Source===
 
===Source===
  
−
Dxs: Escherichia coli
+
Dxs: ''Escherichia coli''
−
LIMS1: Citrus lemon
+
<br>
−
appY: Escherichia coli
+
LIMS1: ''Citrus lemon''
−
 
+
<br>
 +
appY: ''Escherichia coli''
  
 
===References===
 
===References===

Latest revision as of 21:13, 15 October 2014

dxs+LIMS1+appY regulated by the tet promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 808
    Illegal SapI.rc site found at 2068


Design Notes

The part was constructed using standard assembly from: Part: BBa_K1475003 and Part:BBa_K118024


Source

Dxs: Escherichia coli
LIMS1: Citrus lemon
appY: Escherichia coli

References

1. Leighanne A. Brammer et al.: 1-Deoxy-d-xylulose 5-Phosphate Synthase Catalyzes a Novel Random Sequential Mechanism. J Biol Chem. Oct 21, 2011; 286(42): p. 36522–36531. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3196101/
2. BRENDA, 2014: EC 2.2.1.7 - 1-deoxy-D-xylulose-5-phosphate synthase. http://www.brenda-enzymes.org/enzyme.php?ecno=2.2.1.7&Suchword=&organism%5B%5D=Escherichia+coli&show_tm=0
3. BRENDA, 2014: EC 4.2.3.20 - (R)-limonene synthase. http://www.brenda-enzymes.org/enzyme.php?ecno=4.2.3.20&Suchword=&organism%5B%5D=Citrus+limon&show_tm=0
4. UniProt, P05052- APPY_ECOLI, http://www.uniprot.org/uniprot/P05052