Difference between revisions of "Part:BBa K1554006:Design"

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===Design Notes===
 
===Design Notes===
  
−
The sequence that contains the BsmBI restriction sites and the overhangs was ordered as two separate primers to Thermo Scientific.
+
The sequence contains two BsmBI restriction sites, which are type IIs enzymes that cut directionally, leaving two overhangs compatible with GoldenBraid 2.0, a Golden Gate-based assembly system.
  
 
===Source===
 
===Source===
  
−
.
+
This sequence was ordered as two separate primers to Sigma-Aldrich.
  
 
===References===
 
===References===

Latest revision as of 18:22, 15 October 2014


Omega undercover


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence contains two BsmBI restriction sites, which are type IIs enzymes that cut directionally, leaving two overhangs compatible with GoldenBraid 2.0, a Golden Gate-based assembly system.

Source

This sequence was ordered as two separate primers to Sigma-Aldrich.

References