Difference between revisions of "Part:BBa K1554006"
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The omega undercover vector has a BioBrick backbone but is compatible with GoldenBraid 2.0, a Golden Gate-based assembly system. It contains two BsmBI restriction sites which leave the required overhangs to perform a GoldenBraid ligation of two transcriptional units in alpha vectors. We call it "omega undercover" as it works in a similar way of the GoldenBraid 2.0 omega vector but with a pSB1A3 backbone. | The omega undercover vector has a BioBrick backbone but is compatible with GoldenBraid 2.0, a Golden Gate-based assembly system. It contains two BsmBI restriction sites which leave the required overhangs to perform a GoldenBraid ligation of two transcriptional units in alpha vectors. We call it "omega undercover" as it works in a similar way of the GoldenBraid 2.0 omega vector but with a pSB1A3 backbone. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | The omega undercovered rises as a way of trying to make BioBricks compatible with other Golden Gate-based assembly systems. It contains two BsmBI restiction sites which after digestion leave the overhangs required to perform a ligation of two transcriptional units which follow the required grammar. This grammar consists of the sequence GGAG on the 5' end and CGCT on the 3' end. Our team successfully used this vector in order to send [ | + | The omega undercovered rises as a way of trying to make BioBricks compatible with other Golden Gate-based assembly systems. It contains two BsmBI restiction sites which after digestion leave the overhangs required to perform a ligation of two transcriptional units which follow the required grammar. This grammar consists of the sequence GGAG on the 5' end and CGCT on the 3' end. Our team successfully used this vector in order to send [https://parts.igem.org/Part:BBa_K1554004 Part:BBa_K1554004] and [https://parts.igem.org/Part:BBa_K1554005 Part:BBa_K1554005] to the Registry of Parts. The molecular process involved in such ligation is explained in the following figure. |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1554006 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1554006 SequenceAndFeatures</partinfo> | ||
Latest revision as of 18:28, 15 October 2014
Omega undercover
The omega undercover vector has a BioBrick backbone but is compatible with GoldenBraid 2.0, a Golden Gate-based assembly system. It contains two BsmBI restriction sites which leave the required overhangs to perform a GoldenBraid ligation of two transcriptional units in alpha vectors. We call it "omega undercover" as it works in a similar way of the GoldenBraid 2.0 omega vector but with a pSB1A3 backbone.
Usage and Biology
The omega undercovered rises as a way of trying to make BioBricks compatible with other Golden Gate-based assembly systems. It contains two BsmBI restiction sites which after digestion leave the overhangs required to perform a ligation of two transcriptional units which follow the required grammar. This grammar consists of the sequence GGAG on the 5' end and CGCT on the 3' end. Our team successfully used this vector in order to send Part:BBa_K1554004 and Part:BBa_K1554005 to the Registry of Parts. The molecular process involved in such ligation is explained in the following figure.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]