Difference between revisions of "Part:BBa K1342001"
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ZinTp (pYodA) is a promoter which expresses the downstream gene in the presence of cadmium ion.
 
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We assembled ZinTp([http://www.example.com BBa_K1342005]), RBS(BBa_B0034), ApeA(BBa_K1342002), Dter(BBa_0015).
 
  
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ZinTp_AspA contains ZinTp([https://parts.igem.org/Part:BBa_K1342005 BBa_K1342005]), RBS([https://parts.igem.org/Part:BBa_B0034 BBa_B0034]), ApeA([https://parts.igem.org/Part:BBa_K1342002 BBa_K1342002]) and Dter([https://parts.igem.org/Part:BBa_B0015 BBa_B0015]).
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The AspA encoding aspartase that synthesize aspartic acid from fumaric acid and ammonium, is expressed in the presence of cadmium ion.
  
  
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<partinfo>BBa_K1342001 parameters</partinfo>
 
<partinfo>BBa_K1342001 parameters</partinfo>
 
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===TLC Assay===
 
===TLC Assay===
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[[File:TLC_20140929_.jpg|200px|thumb|Analysis of aspartate from strain E.coli K-12/BBa_K1342001 by thin-layer chromatography (TLC) Lane: 1, not addition cadmium to previous culture of synthesis medium (diluted 1:50 by synthesis medium) 2, (diluted 1:100 by synthesis medium) 3, add cadmium (250μM) 4, (diluted 1:50 by synthesis medium) 5, (diluted 1:100 by synthesis medium) 6, (diluted 1:200 by synthesis medium)|none]]
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<br><br>
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[[File:TLC_20140929_.jpg|200px|thumb|fig.1
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<br>
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Analysis of aspartic acid synthesized by <i>E. coli</i> JM109/BBa_K1342001 by thin-layer chromatography (TLC) Lane: 1, 2 no addition into culture (diluted 1:50 and 1:100 by synthesis medium, respectively) 3, additon of cadmium ion (250μM) 4, 5, 6 (diluted 1:50, 1:100 and 1:200 by synthesis medium, respectively)|left]]
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[[File:TLC_intensity_of_the_band.jpg|400px|thumb|center|
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fig.2
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<br>
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Estimation of aspartic acid content in spot
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Each intensity of spots indicating the content of aspartic acid, was estimated by Image J.
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.]]

Latest revision as of 12:53, 17 October 2014

zinTp_AspA


Asp Device image.jpg


ZinTp_AspA contains ZinTp(BBa_K1342005), RBS(BBa_B0034), ApeA(BBa_K1342002) and Dter(BBa_B0015). The AspA encoding aspartase that synthesize aspartic acid from fumaric acid and ammonium, is expressed in the presence of cadmium ion.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 179
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1071
    Illegal AgeI site found at 600
    Illegal AgeI site found at 1452
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1111



TLC Assay

fig.1
Analysis of aspartic acid synthesized by E. coli JM109/BBa_K1342001 by thin-layer chromatography (TLC) Lane: 1, 2 no addition into culture (diluted 1:50 and 1:100 by synthesis medium, respectively) 3, additon of cadmium ion (250μM) 4, 5, 6 (diluted 1:50, 1:100 and 1:200 by synthesis medium, respectively)


fig.2
Estimation of aspartic acid content in spot Each intensity of spots indicating the content of aspartic acid, was estimated by Image J. .