Difference between revisions of "Part:BBa K1469001:Experience"
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| − | Part is user sequenced: no illegal restriction sites and no mutations | + | Part is user sequenced: no illegal restriction sites and no mutations. |
Protein expression was checked by SDS-PAGE and Western Blot.[http://2014.igem.org/Team:Saarland/4_step#WesternBlot] | Protein expression was checked by SDS-PAGE and Western Blot.[http://2014.igem.org/Team:Saarland/4_step#WesternBlot] | ||
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===Applications of BBa_K1469001=== | ===Applications of BBa_K1469001=== | ||
| − | BioBrick is designed for production of high molecular mass hyaluronic acid (HMM-HA) in a ''Bacillus megaterium'' based expression system. HMM-HA is supposed to have inhibitory effects on cancer cell lines | + | BioBrick is designed for production of high molecular mass hyaluronic acid (HMM-HA) in a ''Bacillus megaterium'' based expression system. HMM-HA is supposed to have inhibitory effects on cancer cell lines. |
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 13:37, 15 October 2014
Part is user sequenced: no illegal restriction sites and no mutations.
Protein expression was checked by SDS-PAGE and Western Blot.[http://2014.igem.org/Team:Saarland/4_step#WesternBlot]
Localisation of a GFP tagged Has2 protein variant was checked by fluorescence microscopy.[http://2014.igem.org/Team:Saarland/4_step#Fluorescence]
No catalytic activity probably due to misfolding and intracellular accumulation.[http://2014.igem.org/Team:Saarland/4_step#Rheometer]
Applications of BBa_K1469001
BioBrick is designed for production of high molecular mass hyaluronic acid (HMM-HA) in a Bacillus megaterium based expression system. HMM-HA is supposed to have inhibitory effects on cancer cell lines.
User Reviews
UNIQb7b5b4ae560300b4-partinfo-00000000-QINU UNIQb7b5b4ae560300b4-partinfo-00000001-QINU