Difference between revisions of "Part:BBa K1415206"
Alex19950425 (Talk | contribs) |
Alex19950425 (Talk | contribs) |
||
(6 intermediate revisions by the same user not shown) | |||
Line 2: | Line 2: | ||
<partinfo>BBa_K1415206 short</partinfo> | <partinfo>BBa_K1415206 short</partinfo> | ||
− | We | + | To predict the PBAN expression in E.coli by computer modeling, we next tested PBAN BFP biobricks. We obtained the average expressive value of the blue fluorescence in the biobrick part (above) and also the control part of Pcons + RBS + BFP + Ter. Therefore, we can use the average value to generate predictions of the PBAN expression in E.coli. |
Line 17: | Line 17: | ||
<partinfo>BBa_K1415206 parameters</partinfo> | <partinfo>BBa_K1415206 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
− | [[File:ALLHAH.png| | + | |
− | [[File:BLHAH.png|1200px| | + | |
+ | |||
+ | [[File:ALLHAH.png|thumb|center|1200px|'''Fig.1-1''' Biobrick of Pcons + RBS + PBAN(HAH) + BFP + Term. ]] | ||
+ | [[File:BLHAH.png|thumb|center|1200px|'''Fig.1-2''' The growth curve of E.coli containing Pcons + RBS + PBAN(HAH) + RBS + BFP + Ter plasmid (control is the competent cells which can not emit blue light). ]] | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | [[File:nFLHAH.png|thumb|center|650px|'''Fig.1-3''' The blue light fluorescence expression curve of E.coli containing Pcons + RBS + PBAN(HAH) + RBS + BFP + Ter plasmid (control: competent cells that cannot emit blue light). | ||
+ | ]] | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | [[File:nODHAH2.png|thumb|center|650px|'''Fig.1-4''' The growth curves of PBAN(HAH)]] | ||
+ | |||
+ | |||
+ | |||
+ | [[File:MDHAH2.png|thumb|center|1200px|'''Fig.1-5'''Modeling result of Pcons + RBS + PBAN(HAH) + BFP + Ter. The blue line is the expression profile of the theoretical biobrick. And the green line is the expression data of Pcons + RBS + PBAN(HAH) + BFP + Ter. And the red line is the adjusting line from the green and blue one. This line represent the correcting line of theoretical data and real condition data which can make our model not only fit the theoretical condition but also stay away from experimental bias. ]] |
Latest revision as of 17:14, 25 October 2014
Pcons+B0034+PBAN(Helicoverpa armigera Hubner)+B0034+BFP+J61048
To predict the PBAN expression in E.coli by computer modeling, we next tested PBAN BFP biobricks. We obtained the average expressive value of the blue fluorescence in the biobrick part (above) and also the control part of Pcons + RBS + BFP + Ter. Therefore, we can use the average value to generate predictions of the PBAN expression in E.coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 79
Illegal NgoMIV site found at 909 - 1000COMPATIBLE WITH RFC[1000]