Difference between revisions of "Part:BBa I746352:Experience"
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===User Reviews=== | ===User Reviews=== | ||
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<I>Cooper Union iGEM 2014</I> | <I>Cooper Union iGEM 2014</I> | ||
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| − | We used this part to design a composite part, <partinfo>BBa_K1354000</partinfo>, with this phage activator and a promoter, <partinfo>BBa_K518010</partinfo>. This phage activator turned on a phage promoter with a GFP reporter composite part, <partinfo>BBa_I746321</partinfo>. The two composite parts were in separate plasmids. | + | We used this part to design a composite part, <partinfo>BBa_K1354000</partinfo>, with this phage activator and a promoter, <partinfo>BBa_K518010</partinfo>. This phage activator turned on a phage promoter with a GFP reporter composite part, <partinfo>BBa_I746321</partinfo>. The two composite parts were in separate plasmids. When shocked with UV, the UV promoter was turned on, which activated the phage activator. This then turned the phage promoter on which activated the GFP reporter. The fluorescence readings are shown below. |
[[File:CU_UV_promoter_graph.JPG]] | [[File:CU_UV_promoter_graph.JPG]] | ||
[[File:CU_UV_promoter_table.JPG]] | [[File:CU_UV_promoter_table.JPG]] | ||
Latest revision as of 18:55, 15 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_I746352
User Reviews
UNIQ155fe64f60724e89-partinfo-00000000-QINU
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Cooper Union iGEM 2014 |
We used this part to design a composite part, BBa_K1354000, with this phage activator and a promoter, BBa_K518010. This phage activator turned on a phage promoter with a GFP reporter composite part, BBa_I746321. The two composite parts were in separate plasmids. When shocked with UV, the UV promoter was turned on, which activated the phage activator. This then turned the phage promoter on which activated the GFP reporter. The fluorescence readings are shown below.
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UNIQ155fe64f60724e89-partinfo-00000005-QINU