Difference between revisions of "Part:BBa K1462140:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | This part is constructed as a control group to identify our enzymes have been directed to the mitochondria.The GFP is fused at the C-terminus of Ccr to report where the enzyme located.Under the confocal microscope, we can observe the location of green fluorescence, thus to confirm the exact subcellular localization of target proteins. | |
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| − | + | [[File:GCG.jpg|200px|left|frame|Figure 1. <b>Enzymes located in cytoplasm</b> The fluorescence indicates that Ccr is expressed in the cytoplasm]] | |
Latest revision as of 13:09, 17 October 2014
Gal1+Ccr+GFP+ADH1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1203
Illegal BsaI.rc site found at 2549
Design Notes
This part is constructed as a control group to identify our enzymes have been directed to the mitochondria.The GFP is fused at the C-terminus of Ccr to report where the enzyme located.Under the confocal microscope, we can observe the location of green fluorescence, thus to confirm the exact subcellular localization of target proteins.
Figure 1. Enzymes located in cytoplasm The fluorescence indicates that Ccr is expressed in the cytoplasm