Difference between revisions of "Part:BBa K1524100"

Latest revision as of 16:19, 1 November 2014

H-stem expression vector

This device provides you an easy and strong method to silence mRNA using anti-sense RNA. All you have to do is to insert your target gene sequence upside down using NcoI and XhoI restriction enzymes. Add IPTG, and your target will be repressed! Notice that this stem sequence has a resistance to PCR, especially sequencing PCR. And notice that its vector is pSB1A3 becaouse 1C3 vector has both NcoI site and XhoI site. This part works also as 60 nt of anti-snese for mRFP. (Please refer to BBa_K1524104 etc.)

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal suffix found in sequence at 477
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 342
Illegal SpeI site found at 478
Illegal PstI site found at 492
Illegal NotI site found at 485
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 300
23
INCOMPATIBLE WITH RFC[23]
Illegal suffix found in sequence at 478
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 478
Illegal PstI site found at 492
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 239

@@ Line 5: / Line 5: @@
 All you have to do is to insert your target gene sequence upside down using NcoI and XhoI restriction enzymes.
 Add IPTG, and your target will be repressed!
+Notice that this stem sequence has a resistance to PCR, especially sequencing PCR. And notice that its vector is pSB1A3 becaouse 1C3 vector has both NcoI site and XhoI site.
+This part works also as 60 nt of anti-snese for mRFP. (Please refer to BBa_K1524104 etc.)
 <!-- Add more about the biology of this part here
@@ Line 12: / Line 14: @@
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K1524100 SequenceAndFeatures</partinfo>
 <!-- Uncomment this to enable Functional Parameter display