Difference between revisions of "Part:BBa K1520017"
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It is a high level detection plasmid. It produces rfp when AHL is below a certain level. The following picture shows that without AHL, the bacteria with the plasmid can express rfp. | It is a high level detection plasmid. It produces rfp when AHL is below a certain level. The following picture shows that without AHL, the bacteria with the plasmid can express rfp. | ||
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[[File:withoutAHL.jpg]] | [[File:withoutAHL.jpg]] | ||
| + | [[File:k1520017.jpg]] | ||
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| + | From left to right, Marker(1Kb), Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter, Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter-Plac-rbs-rfp-Ter | ||
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| + | The substances of electrophoresis are made from PCR of plasmids with VF2 and VR as their primers. | ||
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Latest revision as of 00:52, 18 October 2014
Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter-Plac-rbs-rfp-Ter
Plasmid1 in detector: Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter-Plac-rbs-rfp-Ter.
It is a high level detection plasmid. It produces rfp when AHL is below a certain level. The following picture shows that without AHL, the bacteria with the plasmid can express rfp.
From left to right, Marker(1Kb), Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter, Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter-Plac-rbs-rfp-Ter
The substances of electrophoresis are made from PCR of plasmids with VF2 and VR as their primers.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2215
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 985