Difference between revisions of "Part:BBa K1350007"

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<partinfo>BBa_K1350007 short</partinfo>
 
<partinfo>BBa_K1350007 short</partinfo>
  
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we got this endoglucanase from iGEM kit,and we mutate it in a certain spot.[https://parts.igem.org/Part:BBa_K805011]<br>
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===Cel5A, endoglucanases===
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The endoglucanase genes cel5A can hydrolyze β-1,4glucosan randomly and act on long cellulose chains. The main product is oligomeric glucose. It can digest glucosan to produce glucose monomer in high efficiency with synergy with β-1,4glucosidase.It shows high ability to hydrolyze the crystal cellulose but relative lower enzyme activity towards carboxymethyl cellulose, barley β-glucan, and PASC.
 
The endoglucanase genes cel5A can hydrolyze β-1,4glucosan randomly and act on long cellulose chains. The main product is oligomeric glucose. It can digest glucosan to produce glucose monomer in high efficiency with synergy with β-1,4glucosidase.It shows high ability to hydrolyze the crystal cellulose but relative lower enzyme activity towards carboxymethyl cellulose, barley β-glucan, and PASC.
  
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===Usage and Biology===
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===Improved endoglucanase Cel5A===
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Different from BBa_K805011 given by iGEM12_HUST-China, we have done the mutation of an ? to ?? and change the sequence in a way.
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BBa_K805011[https://parts.igem.org/Part:BBa_K805011] given by iGEM12_HUST-China had BamH I site. The site is cleared by introducing a silent mutation. GGATCC (874-879) has been changed to GGGTCC. A codon GGA(Gly) is changed to GGG(Gly). Because of the degeneracy of codon,the different sequence can encode the same enzyme.This  silent mutation makes the part can be compatible with RFC[21].
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However,because of the degeneracy of codon,the different sequence can encode the same enzyme.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1350007 SequenceAndFeatures</partinfo>
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 07:02, 17 October 2014

Cel5A, endoglucanase（BBa_K805011 Improved）


The endoglucanase genes cel5A can hydrolyze β-1,4glucosan randomly and act on long cellulose chains. The main product is oligomeric glucose. It can digest glucosan to produce glucose monomer in high efficiency with synergy with β-1,4glucosidase.It shows high ability to hydrolyze the crystal cellulose but relative lower enzyme activity towards carboxymethyl cellulose, barley β-glucan, and PASC.

Improved endoglucanase Cel5A

BBa_K805011[1] given by iGEM12_HUST-China had BamH I site. The site is cleared by introducing a silent mutation. GGATCC (874-879) has been changed to GGGTCC. A codon GGA(Gly) is changed to GGG(Gly). Because of the degeneracy of codon,the different sequence can encode the same enzyme.This silent mutation makes the part can be compatible with RFC[21].



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 307
    Illegal NgoMIV site found at 763
    Illegal AgeI site found at 1027
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1024