Difference between revisions of "Part:BBa K1381009"

Latest revision as of 15:25, 16 October 2014

yenbox_J23113-B0032-GFP

This construct was created for characterising the quorum sensing system from Yersinia enterocolitica, the Yen system. yenbox_WT is one of the parts in the Yen system, a recognition region (homologous to the luxbox from the well known Lux system), fused with a wildtype promoter. When interaction between the activator BBa_K1381004 (B0034-YenR) and the yenbox occur, the expression level of the fused promoter is induced.

The BioBricks that were used for assembling this construct was BBa_K1381001 (yenbox_J23113) and BBa_E0040 (the green fluorescence protein GFP with the RBS B0032). To read more about the Yen system and for the results from the characterisation experiments, see BBa_K1381004 (B0034-YenR) or BBa_K1381001 (yenbox_J23113).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 41
Illegal NheI site found at 52
Illegal NheI site found at 75
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 751

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−	The ~~BioBrick~~ that were used for assembling this construct was [https://parts.igem.org/Part:~~BBa_K138001 BBa_K138001~~] (yenbox_J23113) and [https://parts.igem.org/Part:BBa_E0040 BBa_E0040] (the green fluorescence protein GFP with the RBS B0032).	+	The BioBricks that were used for assembling this construct was [https://parts.igem.org/Part:BBa_K1381001 BBa_K1381001] (yenbox_J23113) and [https://parts.igem.org/Part:BBa_E0040 BBa_E0040] (the green fluorescence protein GFP with the RBS B0032).
−	~~Too~~ read more about the Yen system and for the results from the characterisation experiments, see [https://parts.igem.org/Part:BBa_K1381004 BBa_K1381004] (B0034-YenR) or [https://parts.igem.org/Part:BBa_K1381001 BBa_K1381001] (yenbox_J23113).	+	To read more about the Yen system and for the results from the characterisation experiments, see [https://parts.igem.org/Part:BBa_K1381004 BBa_K1381004] (B0034-YenR) or [https://parts.igem.org/Part:BBa_K1381001 BBa_K1381001] (yenbox_J23113).