Difference between revisions of "Part:BBa K1520519"
 
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If gold ions exist, rfp will not be produced.
 
If gold ions exist, rfp will not be produced.
  
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[[File:suicidewithoutgold.jpg]]
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this is part of our part BBa_K1520509 [https://parts.igem.org/Part:BBa_K1520509] and the picture is also part of BBa_K1520509, since BBa_K1520509 is based on this one and our result shows it works, this one would definitely work.
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[[File:k1520509.jpg]]
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From left to right, Marker(1Kb), PgolTS-golB-PgolB-tetR-Ter, PgolTS-golB-PgolB-tetR-Ter-Ptet-rbs-rfp-Ter.
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The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 01:53, 18 October 2014

PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter

PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter. If gold ions exist, rfp will not be produced.

Suicidewithoutgold.jpg

this is part of our part BBa_K1520509 [1] and the picture is also part of BBa_K1520509, since BBa_K1520509 is based on this one and our result shows it works, this one would definitely work.

K1520509.jpg

From left to right, Marker(1Kb), PgolTS-golB-PgolB-tetR-Ter, PgolTS-golB-PgolB-tetR-Ter-Ptet-rbs-rfp-Ter.

The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 548
  • 1000
    COMPATIBLE WITH RFC[1000]