Difference between revisions of "Part:BBa K1381018"
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<partinfo>BBa_K1381018 short</partinfo> | <partinfo>BBa_K1381018 short</partinfo> | ||
− | '''USP45''' USP45 is en export tag that works in '' | + | '''USP45'''<br>USP45 is en export tag that works in ''Lactobacillus'' as well as ''E.coli.'' |
− | '''The killing system''' We designed the killing system to secret the colicin Fy with the help of an export tag, | + | '''The killing system'''<br> We designed the killing system to secret the colicin Fy with the help of an export tag, USP45. This part contains the RBS B0034 and the export tag USP45. In team Uppsala iGEM 2014 project this part was to be coupled to the colicin Fy [https://parts.igem.org/Part:BBa_K1381017 BBa_K1381017]. The biobrick suffix is RFC25 and contains an AgeI restriction site for easier fusion with other protein sequences (The colicin Fy [https://parts.igem.org/Part:BBa_K1381017 BBa_K1381017] part was designed with an RFC25 prefix with NgoMIV site). |
Latest revision as of 19:00, 17 October 2014
B0034-USP45-(GS)x5
USP45
USP45 is en export tag that works in Lactobacillus as well as E.coli.
The killing system
We designed the killing system to secret the colicin Fy with the help of an export tag, USP45. This part contains the RBS B0034 and the export tag USP45. In team Uppsala iGEM 2014 project this part was to be coupled to the colicin Fy BBa_K1381017. The biobrick suffix is RFC25 and contains an AgeI restriction site for easier fusion with other protein sequences (The colicin Fy BBa_K1381017 part was designed with an RFC25 prefix with NgoMIV site).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 130
- 1000COMPATIBLE WITH RFC[1000]