Difference between revisions of "Part:BBa K1442102"
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== RGE ==
 
== RGE ==
  
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Reverse GFP for E.coli (RGE) is a specially designed testing module. It contains GFP codon optimised to work in E.coli in the reverse orientation, followed by a ribosome binding site (RBS)……………. After the RBS is the 3’UTR SLD3 containing a ribozyme (BBa_K1442114) which is an RDRP (BBa_K1442100) initiator.
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<p>Reverse GFP for E.coli (RGE) is a specially designed testing module. It contains GFP codon optimised to work in E.coli synthesised as the reverse complement, followed by a ribosome binding site also reversed <html><body><a href="https://parts.igem.org/Part:BBa_B0034">Part BBa_B0034</a></body></html> After the RBS is the 3’UTR SLD3 containing a ribozyme <html><body><a href="https://parts.igem.org/Part:BBa_K1442114">Part BBa_K1442114</a></body></html> which is an RDRP promoter and is the test subject used in this testing module. This can be exchanged to test other promoters using the AflII and BsiWI restriction sites either side.</p>
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<p>Each 3' promoter has a hammerhead ribozyme following it in order to create a clean slice with no scar removing the T7 terminator following transcription.</p> 
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===Usage ===
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[[File:RGE.jpg]]
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''This graphic generated from Geneious shows the organisation of the RGE testing module.
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== Usage ==
 
== Usage ==
  
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The RGE was intended to be used to test the different 3’UTR options which can be cloned in using restriction digest enzymes Afl II and BsiW1.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1442102 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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This testing module is designed to work in RNA format.
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===Functional Parameters===
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<partinfo>BBa_K1442102 parameters</partinfo>
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Latest revision as of 22:14, 15 October 2014

Reverse GFP for E.coli

RGE

Reverse GFP for E.coli (RGE) is a specially designed testing module. It contains GFP codon optimised to work in E.coli synthesised as the reverse complement, followed by a ribosome binding site also reversed Part BBa_B0034 After the RBS is the 3’UTR SLD3 containing a ribozyme Part BBa_K1442114 which is an RDRP promoter and is the test subject used in this testing module. This can be exchanged to test other promoters using the AflII and BsiWI restriction sites either side.

Each 3' promoter has a hammerhead ribozyme following it in order to create a clean slice with no scar removing the T7 terminator following transcription.

RGE.jpg This graphic generated from Geneious shows the organisation of the RGE testing module.

Usage

The RGE was intended to be used to test the different 3’UTR options which can be cloned in using restriction digest enzymes Afl II and BsiW1.

This testing module is designed to work in RNA format.