Difference between revisions of "Part:BBa K1486025"
 
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<partinfo>BBa_K1486025 short</partinfo>
 
<partinfo>BBa_K1486025 short</partinfo>
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The ADH1 terminator (Alcohol dehydrogenase) is a constitutive terminator in the budding yeast S.cerevisae.
 
The ADH1 terminator (Alcohol dehydrogenase) is a constitutive terminator in the budding yeast S.cerevisae.
  
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<FONT SIZE="2"><P>
 
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The ADH1 terminator was extracted by PCR from plasmids we ordered to addgene:Plasmid 44901 pFA6a-link-yoSuperfolderGFP-Kan and Plasmid 44873 pFA6a-link-yoSuperfolderGFP-CaURA3.   
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The ADH1 terminator was extracted by PCR from plasmids we ordered to addgene:Plasmid 44901 pFA6a-link-yoSuperfolderGFP-Kan and Plasmid 44873 pFA6a-link-yoSuperfolderGFP-CaURA3.  A transcription terminator is necessary to avoid mRNA synthesis further than the gene of interest and control which sequence will serve as a 3'UTR, a regulatory subunit of the mRNA.
 
</P></FONT>
 
</P></FONT>
 
'''Secondary Structure'''
 
'''Secondary Structure'''
  
 
[[Image:Mfold-K1486025-1.png]]
 
[[Image:Mfold-K1486025-1.png]]
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<partinfo>BBa_K1486025 SequenceAndFeatures</partinfo>
  
 
<hr>
 
<hr>
 
'''Measurement'''
 
'''Measurement'''
 
* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
 
* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]

Latest revision as of 11:09, 14 October 2014

Terminator.png

biology-NA-
chassisSaccharomyces Cerevisiae
directionForward
functionTerminator

ADH1 Terminator

The ADH1 terminator (Alcohol dehydrogenase) is a constitutive terminator in the budding yeast S.cerevisae.

Purpose and origin of the Biobrick

The ADH1 terminator was extracted by PCR from plasmids we ordered to addgene:Plasmid 44901 pFA6a-link-yoSuperfolderGFP-Kan and Plasmid 44873 pFA6a-link-yoSuperfolderGFP-CaURA3. A transcription terminator is necessary to avoid mRNA synthesis further than the gene of interest and control which sequence will serve as a 3'UTR, a regulatory subunit of the mRNA.

Secondary Structure

File:Mfold-K1486025-1.png


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]