Difference between revisions of "Part:BBa K1481000"
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<partinfo>BBa_K1481000 short</partinfo> | <partinfo>BBa_K1481000 short</partinfo> | ||
− | This part generates a monomeric red fluorescent protein with his-tag for easy protein purification. It is controlled by a pBAD arabinose promoter. It also contains a coding sequence for the AraC protein, crucial for | + | This part generates a monomeric red fluorescent protein with his-tag for easy protein purification. It is controlled by a pBAD arabinose-inducible promoter. It also contains a coding sequence for the AraC protein, crucial for repression of pBAD in absence of arabinose. |
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Latest revision as of 20:28, 10 October 2014
his-mRFP under arabinose promoter
This part generates a monomeric red fluorescent protein with his-tag for easy protein purification. It is controlled by a pBAD arabinose-inducible promoter. It also contains a coding sequence for the AraC protein, crucial for repression of pBAD in absence of arabinose.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961