Difference between revisions of "Part:BBa K1499400:Design"
(→References) |
|||
(3 intermediate revisions by the same user not shown) | |||
Line 6: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
− | The sequence for this gene was | + | The sequence for this gene was obtained from the <i>Polistes dominula</i> genome[1]. We codon-optimized it for expression in <i>E. coli</i> and ordered it for synthesis with BioBrick prefix and suffix from Integrated DNA Technologies. |
+ | |||
+ | <b>Note:</b> This coding part does not contain a promoter, RBS, transcriptional terminator, etc. These elements must be added for proper functionality. | ||
===Source=== | ===Source=== | ||
Line 15: | Line 17: | ||
''Polistes dominula'' genome: | ''Polistes dominula'' genome: | ||
http://goblinx.soic.indiana.edu/PdomGDB | http://goblinx.soic.indiana.edu/PdomGDB | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− |
Latest revision as of 17:12, 24 October 2014
Chitin-binding wasp protein 1 (PdomMRNAr1.2-03231.1)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 143
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence for this gene was obtained from the Polistes dominula genome[1]. We codon-optimized it for expression in E. coli and ordered it for synthesis with BioBrick prefix and suffix from Integrated DNA Technologies.
Note: This coding part does not contain a promoter, RBS, transcriptional terminator, etc. These elements must be added for proper functionality.
Source
Polistes dominula genome.
References
Polistes dominula genome: http://goblinx.soic.indiana.edu/PdomGDB