Difference between revisions of "Part:BBa K1395001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The RFC 10 assembly was used along with the existing biobricks while designing this biobrick. According to the RFC 10 protocol we were restricted to the use of pSB1K3 as the backbone in order to eliminate the possible false clones which was produced as the Part A and Part B was present in the pSB1C3 backbone. To standardise our part, the clone was digested with EcoR1 and Pst1 and ligated with pSB1C3 again. | + | The RFC 10 assembly was used along with the existing biobricks while designing this biobrick. According to the RFC 10 protocol we were restricted to the use of pSB1K3 as the backbone in order to eliminate the possible false clones which was produced as the Part A and Part B was present in the pSB1C3 backbone. To standardise our part, the clone was digested with EcoR1 and Pst1 and ligated with pSB1C3 again. |
| + | [[File:BBa_K1395001_N0x_clone_biobrick_design.PNG|800px|center|alt text]] | ||
| + | [[File:BBa_K1395001_N0x_clone_vector_map.PNG|800px|center|alt text]] | ||
| + | [[File:Agrose_gel.jpg|800px|center|alt text]] | ||
| + | |||
| + | [[File:SDS_1.jpg|800px|center|alt text]] | ||
===Source=== | ===Source=== | ||
Latest revision as of 03:54, 18 October 2014
nrfA gene (Nitrite reductase enzyme) under constitutive promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 360
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The RFC 10 assembly was used along with the existing biobricks while designing this biobrick. According to the RFC 10 protocol we were restricted to the use of pSB1K3 as the backbone in order to eliminate the possible false clones which was produced as the Part A and Part B was present in the pSB1C3 backbone. To standardise our part, the clone was digested with EcoR1 and Pst1 and ligated with pSB1C3 again.
Source
Biobrick BBA_K1153001 (Escherichia coli K-12) and Constitutive Promoter (BBA_J23119).