Difference between revisions of "Part:BBa K1336007:Design"

 
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===Design Notes===
 
===Design Notes===
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This part was designed by ligating our <a href="https://parts.igem.org/Part:BBa_K314103">BBa_K314103</a> with our preexisting part <a href="https://parts.igem.org/Part:BBa_K1336003">BBa_K1336003</a> 
  
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===Source===
 
===Source===
  
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From genomic sequence of Bacillus subtilis; BsDyP gene inserted into a pET-21a (+) expression vector, provided to us by XXX Research Group.
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From genomic sequence of Bacillus subtilis; BsDyP gene inserted into a pET-21a (+) expression vector, provided by the Microbial & Enzyme Technology Lab Research Group, from Instituto de Tecnologia Química e Biológica in Lisbon University.
  
 
===References===
 
===References===

Latest revision as of 17:54, 21 October 2014


LacI Expression Cassette with BsDyP gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1741
    Illegal BsaI site found at 1750
    Illegal BsaI site found at 2716
    Illegal SapI site found at 2146
    Illegal SapI site found at 2504
    Illegal SapI.rc site found at 2660


Design Notes

This part was designed by ligating our BBa_K314103 with our preexisting part BBa_K1336003

Source

From genomic sequence of Bacillus subtilis; BsDyP gene inserted into a pET-21a (+) expression vector, provided by the Microbial & Enzyme Technology Lab Research Group, from Instituto de Tecnologia Química e Biológica in Lisbon University.

References