Difference between revisions of "Part:BBa K1462260"
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<partinfo>BBa_K1462260 short</partinfo> | <partinfo>BBa_K1462260 short</partinfo> | ||
| − | + | This part express the first enzyme ----thiolase. Erg10 is expressed under control of GAL1 promoter and cyc1 terminator. With the help of CoxVI,the enzyme will be transported into mitochondrial matrix after being translated.Together with BBa_K1462270, BBa_K1462280, BBa_K1462290 and BBa_K1462300, this part is components of the n-butanol biosynthesis pathway. | |
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| + | [[File:Pathway.png|200px|left|frame|Figure 1. The n-butanol biosynthetic pathway. Enzymes are from these organisms: Erg10, S. cerevisiae; Hbd, Crt, AdhE2, Clostridium beijerinckii; Ccr, Streptomyces collinus.]] | ||
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Latest revision as of 19:36, 17 October 2014
Gal1+cox6+Erg10+cyc1
This part express the first enzyme ----thiolase. Erg10 is expressed under control of GAL1 promoter and cyc1 terminator. With the help of CoxVI,the enzyme will be transported into mitochondrial matrix after being translated.Together with BBa_K1462270, BBa_K1462280, BBa_K1462290 and BBa_K1462300, this part is components of the n-butanol biosynthesis pathway.
Figure 1. The n-butanol biosynthetic pathway. Enzymes are from these organisms: Erg10, S. cerevisiae; Hbd, Crt, AdhE2, Clostridium beijerinckii; Ccr, Streptomyces collinus.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1378
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000COMPATIBLE WITH RFC[1000]