Difference between revisions of "Part:BBa K1431201"
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| − | A nuclear localization signal or sequence (NLS) is | + | A protein tags with a nuclear localization signal or sequence(NLS) will bind strongly to importin (aka karyopherin), and, together, the complex will move through the nuclear pore.That is very useful to localize protein or make a sign in cell nucleus. |
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| + | We design two NLS which connect by dozens of nucleotides and can be inserted a coding sequence by BbsI site. See the mechanism of BbsI site work in the [https://parts.igem.org/Part:BBa_K1431201:Design design page]. The two NLS in each side of a protein may increase the probability of protein import into the cell nucleus. | ||
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Latest revision as of 01:54, 18 October 2014
NLS, lead protein into the nucleus
A protein tags with a nuclear localization signal or sequence(NLS) will bind strongly to importin (aka karyopherin), and, together, the complex will move through the nuclear pore.That is very useful to localize protein or make a sign in cell nucleus.
We design two NLS which connect by dozens of nucleotides and can be inserted a coding sequence by BbsI site. See the mechanism of BbsI site work in the design page. The two NLS in each side of a protein may increase the probability of protein import into the cell nucleus.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 66
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 88
Illegal NgoMIV site found at 107 - 1000COMPATIBLE WITH RFC[1000]