Difference between revisions of "Part:BBa K1486048"
 
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<partinfo>BBa_K1486048 short</partinfo>
 
<partinfo>BBa_K1486048 short</partinfo>
  
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Calgary's BBa_K339007 ( [[parts.igem.org/Part:BBa_K339007]] ) repaired: the part didn't have the sequence they said they had, it was missing most of the promoter.
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Calgary's BBa_K339007 [https://parts.igem.org/Part:BBa_K339007] repaired: the part didn't have the sequence they said they had, it was missing most of the promoter.
  
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<!-- Add more about the biology of this part here
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=== Biology of the Part ===
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===Usage and Biology===
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E coli have several native sigma factors in the genome that are upregulated during envelope stress. There is also a new regulon that is currently studied widely, called the Cpx regulon, a well characterized periplasmic regulon found in E. coli. The Cpx pathway is involved in maintaining cellular adhesion and keeping periplasmic environment in check. It regulates proteins which are responsible for cellular adhesion and pili formation. The Cpx pathway is activated by factors which cause protein aggregation in the periplasmic space of gram negative bacteria. Extracellular stresses such as exposure to ethanol, change in pH and temperature change induce the activation of the Cpx heat shock regulon. In our BioBrick this should increase the transcription of the RFP which it regulates.
  
 
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<partinfo>BBa_K1486048 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1486048 SequenceAndFeatures</partinfo>
  
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===Usage===
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Excitation: 555 nm Emission: 632 nm
  
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<!-- Uncomment this to enable Functional Parameter display  
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=== Characterisation ===
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<FONT SIZE="2"><P>
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To check if the cells were responding to different kinds of stress we put them in different environments: 40 mM KCl, 80 mM KCl, 5 % ethanol and 10 % ethanol . As a negative control we added PBS to the same cells. As another negative control cells that did not have the RFP coding sequence where  put in the same environments.
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</P></FONT>
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[[File:BBa_K3396007_copy.png]]
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<FONT SIZE="2"><P>
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We see that there is no sensitivity to the stress factors in this conditions.
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</P></FONT>
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<!-- Uncomment this to enable Functional Parameter display
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K1486048 parameters</partinfo>
 
<partinfo>BBa_K1486048 parameters</partinfo>
 
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Latest revision as of 16:10, 17 October 2014

CpxR reporter

Calgary's BBa_K339007 [1] repaired: the part didn't have the sequence they said they had, it was missing most of the promoter.

Biology of the Part

E coli have several native sigma factors in the genome that are upregulated during envelope stress. There is also a new regulon that is currently studied widely, called the Cpx regulon, a well characterized periplasmic regulon found in E. coli. The Cpx pathway is involved in maintaining cellular adhesion and keeping periplasmic environment in check. It regulates proteins which are responsible for cellular adhesion and pili formation. The Cpx pathway is activated by factors which cause protein aggregation in the periplasmic space of gram negative bacteria. Extracellular stresses such as exposure to ethanol, change in pH and temperature change induce the activation of the Cpx heat shock regulon. In our BioBrick this should increase the transcription of the RFP which it regulates.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 636
    Illegal AgeI site found at 748
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage

Excitation: 555 nm Emission: 632 nm


Characterisation

To check if the cells were responding to different kinds of stress we put them in different environments: 40 mM KCl, 80 mM KCl, 5 % ethanol and 10 % ethanol . As a negative control we added PBS to the same cells. As another negative control cells that did not have the RFP coding sequence where put in the same environments.

BBa K3396007 copy.png

We see that there is no sensitivity to the stress factors in this conditions.