Difference between revisions of "Part:BBa K1321162"

Latest revision as of 01:28, 17 October 2014

NiBP driven by LacI promoter

**Important note**: Please be aware we discovered that this part is out of frame, leading to an incorrect translation and read-through of the stop-codon. This also means any subsequent fusion proteins to the C-terminal are out of frame. This is because there is a deletion of a base compared to the equivalent coding sequence from Heliobacter pylori (GenBank CP000012.1 bp1391851-1392033, reverse complement strand). This existed already in BBa_K1151001 from which we cloned our part. We hope that this part will still be useful, since the error could be corrected relatively easily by a team who wishes to it by site-directed-mutagenesis PCR to insert the missing nucleotide.

This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals. Other fusion parts with this metal binding protein can be seen in the table below:

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 230
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1321162 short</partinfo>
-GATC
+'''**Important note**''': Please be aware we discovered that this part is out of frame, leading to an incorrect translation and read-through of the stop-codon. This also means any subsequent fusion proteins to the C-terminal are out of frame. This is because there is a deletion of a base compared to the equivalent coding sequence from ''Heliobacter pylori'' (GenBank CP000012.1 bp1391851-1392033, reverse complement strand). This existed already in [https://parts.igem.org/Parts:BBa_K1151001 BBa_K1151001] from which we cloned our part. We hope that this part will still be useful, since the error could be corrected relatively easily by a team who wishes to it by site-directed-mutagenesis PCR to insert the missing nucleotide.
+This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals. Other fusion parts with this metal binding protein can be seen in the table below: [[File:IC14-NiBP-part-table.PNG]]
 <!-- Add more about the biology of this part here