Difference between revisions of "Part:BBa K1321152"

Revision as of 09:34, 9 October 2014 (view source) Registry (Talk | contribs)		Latest revision as of 04:52, 2 November 2014 (view source) Xen sm (Talk | contribs)
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	<partinfo>BBa_K1321152 short</partinfo>		<partinfo>BBa_K1321152 short</partinfo>
−	GATC	+	Metallothionein fMT (metal-binding peptide) fused N-terminally to CBDcex (a cellulose-binding domain).
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		+	This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below: [[File:IC14-fMT-part-table.PNG|center]]
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		+	Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.
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		+	For reference, the CBDcex binding affinity to bacterial cellulose has been characterised relative to other sfGFP-CBD fusions, please see [https://parts.igem.org/Part:BBa_K1321342 BBa_K1321342] for details.
	<!-- Add more about the biology of this part here		<!-- Add more about the biology of this part here

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Latest revision as of 04:52, 2 November 2014

metallothionein Fmt fused to CBDcex

Metallothionein fMT (metal-binding peptide) fused N-terminally to CBDcex (a cellulose-binding domain).

This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below:

Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.

For reference, the CBDcex binding affinity to bacterial cellulose has been characterised relative to other sfGFP-CBD fusions, please see BBa_K1321342 for details.

Sequence and Features