Difference between revisions of "Part:BBa K1321122"

Revision as of 09:15, 9 October 2014 (view source) Registry (Talk | contribs)		Latest revision as of 04:50, 2 November 2014 (view source) Xen sm (Talk | contribs)
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	__NOTOC__		__NOTOC__
	<partinfo>BBa_K1321122 short</partinfo>		<partinfo>BBa_K1321122 short</partinfo>
−	GATC	+	Metallothionein (metal binding peptide) SmtA fused N-terminally to CBDcex (a cellulose-binding domain).
		+
		+	This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below: [[File:IC14-SmtA-part-table.PNG|center]]
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		+
		+	Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.
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		+	For reference, the CBDcex binding affinity to bacterial cellulose has been characterised relative to other sfGFP-CBD fusions, please see [https://parts.igem.org/Part:BBa_K1321342 BBa_K1321342] for details.
	<!-- Add more about the biology of this part here		<!-- Add more about the biology of this part here
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	<span class='h3bb'>Sequence and Features</span>		<span class='h3bb'>Sequence and Features</span>
−	<partinfo>BBa_K1321122 SequenceAndFeatures</partinfo>	+	<partinfo>BBa_K1321121 SequenceAndFeatures</partinfo>
	<!-- Uncomment this to enable Functional Parameter display		<!-- Uncomment this to enable Functional Parameter display
	===Functional Parameters===		===Functional Parameters===
−	<partinfo>BBa_K1321122 parameters</partinfo>	+	<partinfo>BBa_K1321121 parameters</partinfo>
	<!-- -->		<!-- -->

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Latest revision as of 04:50, 2 November 2014

Smt fused to CBDcex

Metallothionein (metal binding peptide) SmtA fused N-terminally to CBDcex (a cellulose-binding domain).

This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below:

Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.

For reference, the CBDcex binding affinity to bacterial cellulose has been characterised relative to other sfGFP-CBD fusions, please see BBa_K1321342 for details.

Sequence and Features