Difference between revisions of "Part:BBa K1470004:Design"
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+ | <partinfo>BBa_K1470004 short</partinfo> | ||
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+ | <partinfo>BBa_K1470004 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | ===Design Notes=== | ||
+ | |||
+ | Design | ||
+ | |||
+ | |||
+ | |||
+ | ===Source=== | ||
+ | |||
+ | AG Weber | ||
+ | Institute of Biology II / BIOSS Center for Biological Signalling Studies | ||
+ | Albert-Ludwigs-Universität Freiburg | ||
+ | Germany | ||
+ | |||
+ | ===References=== | ||
+ | <p> | ||
+ | [X] Maxam AM, Gilbert W (1980). „Sequencing end-labeled DNA with base-specific chemical cleavages“. Meth. Enzymol. Methods in Enzymology 65 (1): 499–560. [Y] Lowe ME. „Site-specific mutations in the COOH-terminus of placental alkaline phosphatase: a single amino acid change converts a phosphatidylinositol-glycan-anchored protein to a secreted protein“. J Cell Biol. 1992 Feb;116(3):799-807. | ||
+ | </p> |
Latest revision as of 19:55, 16 October 2014
Secreted Alkaline Phosphatase (SEAP)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 209
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 520
Illegal NgoMIV site found at 1489 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 850
Illegal BsaI.rc site found at 1339
Design Notes
Design
Source
AG Weber Institute of Biology II / BIOSS Center for Biological Signalling Studies Albert-Ludwigs-Universität Freiburg Germany
References
[X] Maxam AM, Gilbert W (1980). „Sequencing end-labeled DNA with base-specific chemical cleavages“. Meth. Enzymol. Methods in Enzymology 65 (1): 499–560. [Y] Lowe ME. „Site-specific mutations in the COOH-terminus of placental alkaline phosphatase: a single amino acid change converts a phosphatidylinositol-glycan-anchored protein to a secreted protein“. J Cell Biol. 1992 Feb;116(3):799-807.