Difference between revisions of "Part:BBa K1415203"
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We use this part as reporter gene, it can check whether our PBAN works and quantify its expression. | We use this part as reporter gene, it can check whether our PBAN works and quantify its expression. | ||
| + | [[File:Pcons(J23101) + RBS(B0034) + PBAN(AI) + RBS(B0034) + BFP(K592100) + Ter(J61048) .png|thumb|center|600px|'''Fig.1-1''' Biobrick of Pcons + RBS + PBAN(AI) + BFP + Term.] | ||
| + | [[File:BLAI.png|thumb|center|1200px|'''Fig.1-2''' Fig.1-2 The bluelight test of our E.coli]] | ||
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| + | [[File:PBAN Fluorescence Value (1).jpg|thumb|center|650px|'''Fig.1-3''' The blue light fluorescence expression curve of E.coli containing Pcons + RBS + 9 different kinds of PBAN + RBS + BFP + Ter plasmid (control: competent cells that cannot emit blue light). Below are biobrick serial numbers of the PBAN abbreviations: | ||
| + | SL: BBa_K1415205 BM: BBa_K1415201 MB: BBa_K1415202 | ||
| + | AI: BBa_K1415203 LD: BBa_K1415204 HAH:BBa_K1415206 | ||
| + | AS: BBa_K1415207 SI: BBa_K1415208 AA: BBa_K1415209]] | ||
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| + | <p style="font-size:120%">'''Modeling'''</p> | ||
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| + | [[File:MDAI.png|thumb|center|1200px|'''Fig.1-4''' Modeling result of Pcons + RBS + PBAN(AI) + BFP + Ter. The blue line is the expression profile of the theoretical biobrick. And the green line is the expression data of Pcons + RBS + PBAN(AI) + BFP + Ter. And the red line is the adjusting line from the green and blue one. This line represent the correcting line of theoretical data and real condition data which can make our model not only fit the theoretical condition but also stay away from experimental bias.]] | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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<partinfo>BBa_K1415203 parameters</partinfo> | <partinfo>BBa_K1415203 parameters</partinfo> | ||
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Latest revision as of 14:41, 20 October 2014
Pcons+B0034+PBAN(Agrotis ipsilon)+B0034+BFP+J61048
We use this part as reporter gene, it can check whether our PBAN works and quantify its expression.
[[File:Pcons(J23101) + RBS(B0034) + PBAN(AI) + RBS(B0034) + BFP(K592100) + Ter(J61048) .png|thumb|center|600px|Fig.1-1 Biobrick of Pcons + RBS + PBAN(AI) + BFP + Term.]
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Fig.1-3 The blue light fluorescence expression curve of E.coli containing Pcons + RBS + 9 different kinds of PBAN + RBS + BFP + Ter plasmid (control: competent cells that cannot emit blue light). Below are biobrick serial numbers of the PBAN abbreviations: SL: BBa_K1415205 BM: BBa_K1415201 MB: BBa_K1415202 AI: BBa_K1415203 LD: BBa_K1415204 HAH:BBa_K1415206 AS: BBa_K1415207 SI: BBa_K1415208 AA: BBa_K1415209
Modeling
Fig.1-4 Modeling result of Pcons + RBS + PBAN(AI) + BFP + Ter. The blue line is the expression profile of the theoretical biobrick. And the green line is the expression data of Pcons + RBS + PBAN(AI) + BFP + Ter. And the red line is the adjusting line from the green and blue one. This line represent the correcting line of theoretical data and real condition data which can make our model not only fit the theoretical condition but also stay away from experimental bias.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 909
- 1000COMPATIBLE WITH RFC[1000]