Difference between revisions of "Part:BBa J63008"

 
 
Line 5: Line 5:
 
yeast codon-optimized nuclear localization sequence from simian virus 40
 
yeast codon-optimized nuclear localization sequence from simian virus 40
  
−
<!-- Add more about the biology of this part here
+
=IISER_BHOPAL 2021=
 
===Usage and Biology===
 
===Usage and Biology===
 +
The nuclear import of plasmid DNA mediated by NLS from SV40 T antigen is a two-step process. The first step involves targeting of import substance to the cytoplasmic side of the nuclear membrane. The process is energy independent and can occur at 0°C. The second step is translocation which is energy and temperature-dependent. It has been found that chelating cytosolic Ca2+ with BAPTA inhibit the DNA-NLS import, and DNA-NLS import is competitively inhibited by BSA-NLS conjugates, reinforcing the specificity of NLS in directing the nuclear import of DNA-NLS complexes[1].
 +
 +
===References===
 +
 +
1.Collas, P., & Aleström, P. (1996). Nuclear localization signal of SV40 T antigen directs import of plasmid DNA into sea urchin male pronuclei in vitro. Molecular Reproduction and Development, 45(4), 431–438.
 +
 +
  
−
<!-- -->
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_J63008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_J63008 SequenceAndFeatures</partinfo>

Latest revision as of 14:59, 9 October 2021


SV40 nuclear localization sequence from SV40; yeast codon optimized

yeast codon-optimized nuclear localization sequence from simian virus 40

IISER_BHOPAL 2021

Usage and Biology

The nuclear import of plasmid DNA mediated by NLS from SV40 T antigen is a two-step process. The first step involves targeting of import substance to the cytoplasmic side of the nuclear membrane. The process is energy independent and can occur at 0°C. The second step is translocation which is energy and temperature-dependent. It has been found that chelating cytosolic Ca2+ with BAPTA inhibit the DNA-NLS import, and DNA-NLS import is competitively inhibited by BSA-NLS conjugates, reinforcing the specificity of NLS in directing the nuclear import of DNA-NLS complexes[1].

References

1.Collas, P., & Aleström, P. (1996). Nuclear localization signal of SV40 T antigen directs import of plasmid DNA into sea urchin male pronuclei in vitro. Molecular Reproduction and Development, 45(4), 431–438.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]