Difference between revisions of "Part:BBa K1321361:Design"

 
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===Design Notes===
 
===Design Notes===
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The fusion protein was cloned together using the NgoMIV and AgeI sites.
 
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The promotor was cloned onto the protein via the XbaI/SpeI sites.
  
 
===Source===
 
===Source===
  
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CBDcenA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone.
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Our NiBP came from BBa_K1151001.
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Our LacI promotor came from BBa_J04500.
  
 
===References===
 
===References===

Latest revision as of 20:04, 24 October 2014


CBDcenA with linker fused to NiBP driven by LacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 230
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The fusion protein was cloned together using the NgoMIV and AgeI sites.

The promotor was cloned onto the protein via the XbaI/SpeI sites.

Source

CBDcenA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone.

Our NiBP came from BBa_K1151001.

Our LacI promotor came from BBa_J04500.

References