Difference between revisions of "Part:BBa K1465227:Design"

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===Design Notes===
 
===Design Notes===
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Classical [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#BioBrick Suffix Insertion] of the sRNA:pfkA containing the anti-sense RNA for the phosphofructokinase (pfkA) of ''Escherichia coli'' (<bbpart>BBa_K1465225</bbpart>) into the pSB1C3 backbone of the T7 promotor (<bbpart>BBa_I719005</bbpart>).
  
 
===Source===
 
===Source===

Latest revision as of 02:20, 8 October 2014


sRNA of phosphofructokinase A under the control of T7 promoter (T7_sRNA:pfkA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 135
    Illegal XhoI site found at 141
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Classical [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#BioBrick Suffix Insertion] of the sRNA:pfkA containing the anti-sense RNA for the phosphofructokinase (pfkA) of Escherichia coli (BBa_K1465225) into the pSB1C3 backbone of the T7 promotor (BBa_I719005).

Source

BBa_K1465225: sRNA:pfkA.
BBa_I719005: T7 promoter

References