Difference between revisions of "Part:BBa K1465225:Design"

Latest revision as of 17:24, 8 October 2014

Knock-down of phosphofructokinase A using sRNA (sRNA:pfkA)

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 104
Illegal XhoI site found at 110
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

Amplification of the pSB1C3-Backbone (BBa_J04450) using the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_sRNApfkA pSB1C3_pre_sRNApfkA] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_sRNApfkA pSB1C3_suf_sRNApfkA]:

pSB1C3_pre_sRNApfkA (43 bp)

5'-TGTGGCTAAAAGAATTAGTACTCTAGAAGCGGCCGCGAATTCC-3'

pSB1C3_suf_sRNApfkA (43 bp)

5'-TGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGTC-3'

[http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson Gibson-Assembly] of the pSB1C3-Backbone and the gBlock containing the antisense RNA of the Phosphofructoskinase (pfkA) of Escherichia coli.

Source

Gensynthesis ([http://eu.idtdna.com/pages/products/genes/gblocks-gene-fragments gBlocks]) of the sRNA with the anti-sense RNA for the phosphofructokinase (pfkA) of Escherchia coli.

References

Dokyun Na, Seung Min Yoo, Hannah Chung, Hyegwon Park, Jin Hwan Park and Sang Yup Lee (2013) Metabolic engineering of

Escherichia coli using synthetic small regulatory RNAs [http://www.nature.com/nbt/journal/v31/n2/pdf/nbt.2461.pdf Nature Biotechnology 31:170 - 174.]

@@ Line 22: / Line 22: @@
 ===References===
+* Dokyun Na, Seung Min Yoo, Hannah Chung, Hyegwon Park, Jin Hwan Park and Sang Yup Lee (2013) Metabolic engineering of
+''Escherichia coli'' using synthetic small regulatory RNAs [http://www.nature.com/nbt/journal/v31/n2/pdf/nbt.2461.pdf ''Nature Biotechnology 31:170 - 174.'']