Difference between revisions of "Part:BBa K1321091"

 
 
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<partinfo>BBa_K1321091 short</partinfo>
 
<partinfo>BBa_K1321091 short</partinfo>
  
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PhytoC+CBD4. TBC
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Synthetic phytochelatin EC20 (metal binding peptide) fused N-terminally to CBDclos (a cellulose-binding domain)
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This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below: [[File:IC14-PC-part-table.PNG]]
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Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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<p> For reference, the cellulose binding domain binding capability of CBDclos (C-terminally fused) to bacterial cellulose was measured relative to other cellulose binding domains when fused to sfGFP, the data for which can be seen ([https://parts.igem.org/Part:BBa_K1321341 here])  - K1321341.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
 
<partinfo>BBa_K1321091 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1321091 SequenceAndFeatures</partinfo>
  

Latest revision as of 06:11, 2 November 2014

Phytochelatin (PC) EC20 fused to CBDclos

Synthetic phytochelatin EC20 (metal binding peptide) fused N-terminally to CBDclos (a cellulose-binding domain)

This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below: IC14-PC-part-table.PNG

Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.

Usage and Biology

For reference, the cellulose binding domain binding capability of CBDclos (C-terminally fused) to bacterial cellulose was measured relative to other cellulose binding domains when fused to sfGFP, the data for which can be seen (here) - K1321341.

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 133
  • 1000
    COMPATIBLE WITH RFC[1000]