Difference between revisions of "Part:BBa K1371040"

Latest revision as of 01:58, 25 October 2014

DEBS1(KR1)

Standardization of Interchangeable Domains

The standardization of sufficient numbers of domains from numerous organisms would allow customized replacement, rearrangement, and/or recombining of different domains to produce novel polyketides. In this part, we tested customized synthesis of triketide lactones by swapping KR domains between modules to test the compatibility of the standardized domains.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1876
Illegal BamHI site found at 6423
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 556
Illegal NgoMIV site found at 2039
Illegal NgoMIV site found at 2411
Illegal NgoMIV site found at 2444
Illegal NgoMIV site found at 2917
Illegal NgoMIV site found at 3626
Illegal NgoMIV site found at 4499
Illegal AgeI site found at 367
Illegal AgeI site found at 1729
Illegal AgeI site found at 2075
Illegal AgeI site found at 2654
Illegal AgeI site found at 4375
Illegal AgeI site found at 4445
Illegal AgeI site found at 5072
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1481
Illegal BsaI.rc site found at 3911
Illegal SapI.rc site found at 2746

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1371040 short</partinfo>
-aa
+== Standardization of Interchangeable Domains ==
+The standardization of sufficient numbers of domains from numerous organisms would allow customized replacement, rearrangement, and/or recombining of different domains to produce novel polyketides. In this part, we tested customized synthesis of triketide lactones by swapping KR domains between modules to test the compatibility of the standardized domains.
+<div class="center">
+[[File:Pict10.png]]
+[[File:Pict12.png]]
+</div>
 <!-- Add more about the biology of this part here
 ===Usage and Biology===