Difference between revisions of "Part:BBa K1455006"
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<partinfo>BBa_K1455006 short</partinfo> | <partinfo>BBa_K1455006 short</partinfo> | ||
| − | ... | + | This device is a positive feedback for itself, producing more amilCP (blue chromoprotein) each time. |
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| + | AmcilCP and PSP3 are expressing constitutively by pTet promoter, which can be turned off by the prescence of TetR wtih no tetracycline. PSP3 is an activator for psid promoter, which is upstream pTet, thus activates AmilCP and PSP3 again and again. | ||
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| + | In addition, PSP3 does not only activate genes downstream of psid, it also serves as activator for other five promoter of the same family (parts BBa_I746360 to BBa_I746365). | ||
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| + | Is important to highlight that TetR is, theoretically, able to turn off all the system, and after the system can be turned on again by the addition of tetracycline (or analogues), so this device could be useful as a part of a more complex regulation circuit. | ||
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| + | Here is an example of an ''E: coli'' DH5α culture carrying this device: | ||
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| + | [[File:partepsid.jpg|400px]] | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 01:21, 10 October 2014
psid - pTet - AmilCP - pag activator (PSP3) Positive feedback
This device is a positive feedback for itself, producing more amilCP (blue chromoprotein) each time.
AmcilCP and PSP3 are expressing constitutively by pTet promoter, which can be turned off by the prescence of TetR wtih no tetracycline. PSP3 is an activator for psid promoter, which is upstream pTet, thus activates AmilCP and PSP3 again and again.
In addition, PSP3 does not only activate genes downstream of psid, it also serves as activator for other five promoter of the same family (parts BBa_I746360 to BBa_I746365).
Is important to highlight that TetR is, theoretically, able to turn off all the system, and after the system can be turned on again by the addition of tetracycline (or analogues), so this device could be useful as a part of a more complex regulation circuit.
Here is an example of an E: coli DH5α culture carrying this device:
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]