Difference between revisions of "Part:BBa K1498001:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K1498001 short</partinfo>
 
<partinfo>BBa_K1498001 short</partinfo>
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===Design Notes===
 
===Design Notes===
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to be filled in
 
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 +
Codon optimised for ''E. coli''.
  
 
===Source===
 
===Source===
  
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Sourced from the BioBricks website, BBa_K258006 was synthesised as it was not available from the catalogue, BBa_J23110 was retrieved from the distribution kit
+
Sourced from the BioBricks website, BBa_K258006 was synthesised as it was not available from the catalogue (but codon optimised for use in ''E. coli'')
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 +
BBa_J23110 was retrieved from the distribution kit
  
 
===References===
 
===References===
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 +
Chung, C. W., You, J., Kim, K., Moon, Y., Kim, H., & Ahn, J. H. (2009). Export of recombinant proteins in Escherichia coli using ABC transporter with an attached lipase ABC transporter recognition domain (LARD). Microbial cell factories, 8(1), 11. doi:10.1186/1475-2859-8-11

Latest revision as of 17:59, 17 October 2014

constitutive promoter with lipase (BBa_J23110, BBa_K258006)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 692
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised for E. coli.

Source

Sourced from the BioBricks website, BBa_K258006 was synthesised as it was not available from the catalogue (but codon optimised for use in E. coli)

BBa_J23110 was retrieved from the distribution kit

References

Chung, C. W., You, J., Kim, K., Moon, Y., Kim, H., & Ahn, J. H. (2009). Export of recombinant proteins in Escherichia coli using ABC transporter with an attached lipase ABC transporter recognition domain (LARD). Microbial cell factories, 8(1), 11. doi:10.1186/1475-2859-8-11