Difference between revisions of "Part:BBa K1442106"

Revision as of 13:39, 7 October 2014 (view source) Registry (Talk | contribs)		Latest revision as of 22:57, 15 October 2014 (view source) CarriedC (Talk | contribs) (→‎FG)
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	__NOTOC__		__NOTOC__
	<partinfo>BBa_K1442106 short</partinfo>		<partinfo>BBa_K1442106 short</partinfo>
−	Forward GFP for use in human cell tests.
−	<!-- Add more about the biology of this part here	+	== FG ==
−	===Usage and Biology===	+
		+	<p>The forward GFP testing module for human cells is a specially designed testing module. It contains GFP codon optimised to work in human cells using IDT codon optimiser and was used to test the EMCV IRES, NKRF IRES and Anti-theophylline aptazyme in vivo.<p>
		+
		+	[[File:FG.jpg]]
−	<!-- -->	+	''This schematic developed in Geneious shows the organisation of the FG testing module''
−	<span class='h3bb'>Sequence and Features</span>	+
−	<partinfo>BBa_K1442106 SequenceAndFeatures</partinfo>	+
		+	== Usage ==
−	<!-- Uncomment this to enable Functional Parameter display	+	The FG was intended to be used to test the EMCV IRES (BBa_K1442023) and the NKRF IRES (BBa_K1442024) using the restriction sites AscI and BsrGI before the GFP. After the GFP are two more restriction sites Mau BI and Mlu I that can be used to test the Aptazyme (BBa_K1442006) and the DPP-IV target (BBa_K1442111).
−	===Functional Parameters===	+
−	<partinfo>BBa_K1442106 parameters</partinfo>	+
−	<!-- -->	+

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Latest revision as of 22:57, 15 October 2014

Forward GFP for human cells

FG

The forward GFP testing module for human cells is a specially designed testing module. It contains GFP codon optimised to work in human cells using IDT codon optimiser and was used to test the EMCV IRES, NKRF IRES and Anti-theophylline aptazyme in vivo.<p> This schematic developed in Geneious shows the organisation of the FG testing module

Usage

The FG was intended to be used to test the EMCV IRES (BBa_K1442023) and the NKRF IRES (BBa_K1442024) using the restriction sites AscI and BsrGI before the GFP. After the GFP are two more restriction sites Mau BI and Mlu I that can be used to test the Aptazyme (BBa_K1442006) and the DPP-IV target (BBa_K1442111).