Difference between revisions of "Part:BBa K1526007"
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<partinfo>BBa_K1526007 short</partinfo> | <partinfo>BBa_K1526007 short</partinfo> | ||
− | + | ''MntH'' gene under control of Lac Repressor System | |
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+ | <b>Gene:</b> mntH<br> | ||
+ | <b>Organism:</b> <i>Escherichia Coli</i><br> | ||
+ | <b>Protein:</b> ''MntH'' transporter from NRAMP family<br> | ||
+ | <b>Function:</b> NRAMP is a membrane divalent metal-ion transporter. In addition to iron and manganese, it also transports Cd ions into the cell.<br> | ||
+ | <b>Aim:</b> We want to use the endogenous transporter NRAMP to take up the cadmium from the environment. The cadmium will then activate pYoda and set off the phytochelatin synthesis process.<br> | ||
+ | <b>Expression:</b> We can run assays measuring the concentration of cadmium in the environment. If ''MntH'' is active, the concentration of cadmium should decrease. | ||
+ | <b>Mutant strains:</b> Information about mutant ''E. coli'' strains for ''MntH'' can be found at EchoBase website from the University of York (http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB3909). | ||
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Latest revision as of 15:35, 18 October 2014
LacRS + MntH
MntH gene under control of Lac Repressor System
Gene: mntH
Organism: Escherichia Coli
Protein: MntH transporter from NRAMP family
Function: NRAMP is a membrane divalent metal-ion transporter. In addition to iron and manganese, it also transports Cd ions into the cell.
Aim: We want to use the endogenous transporter NRAMP to take up the cadmium from the environment. The cadmium will then activate pYoda and set off the phytochelatin synthesis process.
Expression: We can run assays measuring the concentration of cadmium in the environment. If MntH is active, the concentration of cadmium should decrease.
Mutant strains: Information about mutant E. coli strains for MntH can be found at EchoBase website from the University of York (http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB3909).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1188
Illegal BamHI site found at 2462 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2358
- 1000COMPATIBLE WITH RFC[1000]