Difference between revisions of "Part:BBa K1336006:Design"

 
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===Design Notes===
 
===Design Notes===
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When designing PCR primers to change the reverse complement sequence of the ispB gene into the BioBrick format, we truncated the fragment to avoid an illegal PstI restriction site (and so no extra primers were required for site-directed mutagenesis to remove illegal sites).
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This part was designed by ligating our <a href="https://parts.igem.org/Part:BBa_K314103">BBa_K314103</a> with our preexisting part <a href="https://parts.igem.org/Part:BBa_K1336005">BBa_K1336005</a> 
  
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===Source===
 
===Source===

Latest revision as of 16:42, 21 October 2014


LacI Expression Cassette with ispB RC gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed by ligating our BBa_K314103 with our preexisting part BBa_K1336005

Source

From genomic sequence of Escherichia coli.

References