Difference between revisions of "Part:BBa K1351038:Design"

 
 
(5 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1351038 short</partinfo>
 
<partinfo>BBa_K1351038 short</partinfo>
Line 13: Line 12:
 
===Source===
 
===Source===
  
−
artificially synthesized
+
This part was generated by amplification from artificially synthesized DNA with the primers listed below, followed by digestion with EcoRI and PstI and ligation into pSB1C3.
 +
 
 +
P3_fwd: GATCGAATTCGCGGCCGCTTCTAGAG'''GTACAATCTTTTATCATTATATTGCCTAAC'''
 +
 
 +
P3_rev: GATCACTAGTA'''CTCTGTGATCTAGTTATATTAAAACATGC'''
 +
 
 +
Annealing sequences are written in bold.
  
 
===References===
 
===References===
 +
Reynolds, J. and S. Wigneshweraraj (2011). "Molecular Insights into the Control of Transcription Initiation at the Staphylococcus aureus agr operon." Journal of Molecular Biology 412(5): 862-881.

Latest revision as of 21:29, 16 October 2014

P3-promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Several mutated illegal restriction sites. The mutated codons have been optimized regarding the codon usage of Bacillus subtilis.


Source

This part was generated by amplification from artificially synthesized DNA with the primers listed below, followed by digestion with EcoRI and PstI and ligation into pSB1C3.

P3_fwd: GATCGAATTCGCGGCCGCTTCTAGAGGTACAATCTTTTATCATTATATTGCCTAAC

P3_rev: GATCACTAGTACTCTGTGATCTAGTTATATTAAAACATGC

Annealing sequences are written in bold.

References

Reynolds, J. and S. Wigneshweraraj (2011). "Molecular Insights into the Control of Transcription Initiation at the Staphylococcus aureus agr operon." Journal of Molecular Biology 412(5): 862-881.