Difference between revisions of "Part:BBa K1510010"

 
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1510010 short</partinfo>
 
<partinfo>BBa_K1510010 short</partinfo>
  
−
This part contains a strong promoter, J23100, and signal sequence yebF together with is recombinant red florescent protein, and a double terminator at the end. In our experiment, we use the circuit to compare and test if the signal sequence works.
+
Improvements to BBa_K1190003 of 2013 Arizona Team, this design is made to do functional measurement checking YebF,
 +
this part contains a strong promoter, J23100, and signal sequence yebF together with is recombinant red florescent protein, and a double terminator at the end. In our experiment, we use the circuit to compare and test if the signal sequence works.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 18:29, 17 October 2014

Extracellular RFP

Improvements to BBa_K1190003 of 2013 Arizona Team, this design is made to do functional measurement checking YebF, this part contains a strong promoter, J23100, and signal sequence yebF together with is recombinant red florescent protein, and a double terminator at the end. In our experiment, we use the circuit to compare and test if the signal sequence works.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 161
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 965
    Illegal AgeI site found at 1077
  • 1000
    COMPATIBLE WITH RFC[1000]