Difference between revisions of "Part:BBa K1510009:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K1510009=== | ===Applications of BBa_K1510009=== | ||
| − | + | Using yebF at the N-terminal of desired sequence as recombinant protein would help exporting it extracellularly. Especially useful when common experimental strains, such as K-12 are used, since they don't naturally secrete protein outside. | |
===User Reviews=== | ===User Reviews=== | ||
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| + | To test if our yebF works, we fuse RFP at the C-terminal of the protein, and test if the signal protein works by examing the fluorescence intensity in the culture supernatant. | ||
| + | This is our result under Multimode microplate reader: | ||
| + | [[File:NYMU yebF fluroescence intensity of RFP in supernat partial enlargement detail dataJPEG.jpg]] | ||
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Latest revision as of 18:07, 17 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1510009
Using yebF at the N-terminal of desired sequence as recombinant protein would help exporting it extracellularly. Especially useful when common experimental strains, such as K-12 are used, since they don't naturally secrete protein outside.
User Reviews
UNIQ52143479d43fe989-partinfo-00000000-QINU
To test if our yebF works, we fuse RFP at the C-terminal of the protein, and test if the signal protein works by examing the fluorescence intensity in the culture supernatant.
This is our result under Multimode microplate reader:
UNIQ52143479d43fe989-partinfo-00000001-QINU