Difference between revisions of "Part:BBa K1351032"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1351032 short</partinfo>
 
<partinfo>BBa_K1351032 short</partinfo>
  
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"This part was generated in a modified version of RFC25, and has the following prefix and suffix:  
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This part was generated in RFC10, and has the following prefix and suffix:  
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              Prefix                                                                  Suffix
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5' - GAATTC GCGGCCGC T TCTAGA G aagaggagata T ACTAGT A GCGGCCG CTGCAG - 3'
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      EcoRI    NotI      XbaI                                        SpeI    NotI  PstI
  
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{|
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|prefix with EcoRI, NotI, XbaI, SD and NgoMIV:
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This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].
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|<span style=""color:blue"">GAATTC</span><span style=""color:green"">GCGGCCGC</span>T<span style=""color:red"">TCTAGA</span>TG<span style=""color:orange"">GCCGGC</span>
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This library was generated utilizing the [https://salis.psu.edu/software/ Salis lab RBS calculator] to find RBS that cover a high space of translation intitiation rate in 'B. subtilis'. It includes seven RBS with the following sequences:
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{| class=wikitable
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|-
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!Number
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!BBa_K1310 ..
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!Sequence
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|-
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|1
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|28
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|aaggagggata
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|-
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|2
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|29
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|agaggaggata
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|-
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|3
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|30
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|aaggagagata
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|-
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|4
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|31
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|aggagaggata
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|-
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|<b>5</b>
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|<b>32</b>
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|<b>aagaggagata</b>
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|-
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|6
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|33
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|agaaagggata
 
|-
 
|-
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|suffix with AgeI, SpeI, NotI and PstI:
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|7
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|<span style=""color:orange"">ACCGGT</span><u>TAA</u>T<span style=""color:red"">AC<u>TAG</u><u>T</u></span><u>A</u><span style=""color:green""><u>G</u>CGGCCG</span><span style=""color:blue"">CTGCAG</span>
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|34
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|aagaagagata
 
|}
 
|}
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Sites of restriction enzymes generating compatible overhangs have the same color:
 
  
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<span style=""color:blue"">EcoRI</span> and <span style=""color:blue"">PstI</span> in blue, <span style=""color:green"">NotI</span> in green, <span style=""color:red"">XbaI</span> and <span style=""color:red"">SpeI</span> in red, <span style=""color:orange"">NgoMIV</span> and <span style=""color:orange"">AgeI</span> in orange. Stop codons are underlined."
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The coverage of RBS strength as calculated by the [https://salis.psu.edu/software/ RBS calculator] is depicted in the Figure 1 shown below. It shows an evenly distributed strength spanning approxiamately three orders of magnitude.
  
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[[File:LMU_14_RBSlibrary.png|thumb|center|400px|Fig.1: Coverage of the translation initiation rate of the RBS library as calculated by the [https://salis.psu.edu/software/ RBS calculator]]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 06:39, 1 November 2014

LMU Bacillus RBS collection 5

This part was generated in RFC10, and has the following prefix and suffix: 

             Prefix                                                                   Suffix
5' - GAATTC GCGGCCGC T TCTAGA G aagaggagata T ACTAGT A GCGGCCG CTGCAG - 3'
     EcoRI    NotI      XbaI                                         SpeI     NotI   PstI


This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus]. This library was generated utilizing the Salis lab RBS calculator to find RBS that cover a high space of translation intitiation rate in 'B. subtilis'. It includes seven RBS with the following sequences:

Number BBa_K1310 .. Sequence
1 28 aaggagggata
2 29 agaggaggata
3 30 aaggagagata
4 31 aggagaggata
5 32 aagaggagata
6 33 agaaagggata
7 34 aagaagagata

The coverage of RBS strength as calculated by the RBS calculator is depicted in the Figure 1 shown below. It shows an evenly distributed strength spanning approxiamately three orders of magnitude.

Fig.1: Coverage of the translation initiation rate of the RBS library as calculated by the RBS calculator

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]