Difference between revisions of "Part:BBa K1513002:Design"

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. merR mechanism
 
. merR mechanism
 
. why we add a merR to a existed part?(advantage,necessity)
 
. why we add a merR to a existed part?(advantage,necessity)
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 +
 +
 +
 +
We built our part with a existed part (BBa_K346002)----which provided us a PmerT promoter----and a merR sequence synthetized by ourselves. However, you need to add upstream an rbs to make it work if you decide to adopt our part.
  
 
===Source===
 
===Source===
−
rbs: PartBBa_E0240
+
 
 
PmerT: PartBBa_K346002
 
PmerT: PartBBa_K346002
−
merR; we synthesized it by ourselves.
+
 
 +
merR: we synthesized it by ourselves.
  
 
===References===
 
===References===
 
Hobman, J. L., J. Wilkie, et al. (2005). "A design for life: prokaryotic metal-binding MerR family regulators." Biometals 18(4): 429-436.
 
Hobman, J. L., J. Wilkie, et al. (2005). "A design for life: prokaryotic metal-binding MerR family regulators." Biometals 18(4): 429-436.

Latest revision as of 15:28, 8 October 2014


Hg(II) sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1184


Design Notes

. merR mechanism . why we add a merR to a existed part?(advantage,necessity)



We built our part with a existed part (BBa_K346002)----which provided us a PmerT promoter----and a merR sequence synthetized by ourselves. However, you need to add upstream an rbs to make it work if you decide to adopt our part.

Source

PmerT: PartBBa_K346002

merR: we synthesized it by ourselves.

References

Hobman, J. L., J. Wilkie, et al. (2005). "A design for life: prokaryotic metal-binding MerR family regulators." Biometals 18(4): 429-436.