Difference between revisions of "Part:BBa K1412002"
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| − | =''' | + | ='''Composite part of BioBrick [https://parts.igem.org/Part:BBa_K1412001 BBa_K1412001] and [https://parts.igem.org/Part:BBa_K1412033 BBa_K1412033]'''= |
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| − | == What it is == | + | == '''What it is''' == |
| − | + | Composite part of BioBrick [https://parts.igem.org/Part:BBa_K1412001 BBa_K1412001] and [https://parts.igem.org/Part:BBa_K1412033 BBa_K1412033] with promoter removed. | |
| − | == What it does == | + | == '''What it does''' == |
| − | + | Ligating this part with different promoters such as pBAD, pTET, pCons and transform the plasmid into ''E.coli''(''CL-1''), we can endow the bacteria the ability of responding to different inducers and inhibitors. On the basis of this mechanism, we could take the ''E.coli''(''CL-1'') under our command to form different patterns which has mathematically meanings. | |
| + | == '''How to use it''' == | ||
| − | + | To form ellipse, we ligate this part with promoter pCons and transform the plasmid into ''E.coli''(''CL-1''), then we cultivate the bacteria on the plate with M63 medium by controlling the concentration of IPTG and chloromycetin. Doting IPTG on the plate is the most critical procedure on the whole course. By tracking and measuring the chemotaxis diameter of the bacteria on the plate, we get a series of experimental data, More detail in Experimental Data. | |
| − | + | ||
| + | To form hyperbolic curve and parabola, we use promoter pBAD which is specifically recognized by L-Arabinose(pTET which is specially recognized by anhydrotetracycline). Then we cultivate the bacteria on the plate with M63 medium controlling the concentrations of IPTG, L-Arabinose (anhydrotetracycline) and chloromycetin. After the M63 medium dries up, we dot the IPTG and L-Arabinose (anhydrotetracycline) on the plate to form the mathematically meaningful pattern. By tracking and measuring the chemotaxis diameter of the bacteria on the plate, we get a series of experimental data, more detail in Experimental Data. | ||
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===With K20600(pBAD) as an inhibit promoter=== | ===With K20600(pBAD) as an inhibit promoter=== | ||
| − | [ | + | [https://parts.igem.org/Part:BBa_K1412001 The characterization results of part BBa_K1412001] |
| + | ===With R0040(pTET) as an inhibit promoter=== | ||
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| − | + | [https://parts.igem.org/Part:BBa_K1412033 The characterization results of part BBa_K1412033] | |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
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<span class='h3bb'> | <span class='h3bb'> | ||
| − | == Sequence and Features == | + | == '''Sequence and Features''' == |
</span> | </span> | ||
<partinfo>BBa_K1412002 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1412002 SequenceAndFeatures</partinfo> | ||
| + | ---- | ||
| + | <I><B>More information, click here: [http://2014.igem.org/Team:XMU-China# XMU-China] | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K1412002 parameters</partinfo> | <partinfo>BBa_K1412002 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
Latest revision as of 17:57, 14 October 2014
Contents
Composite part of BioBrick BBa_K1412001 and BBa_K1412033
What it is
Composite part of BioBrick BBa_K1412001 and BBa_K1412033 with promoter removed.
What it does
Ligating this part with different promoters such as pBAD, pTET, pCons and transform the plasmid into E.coli(CL-1), we can endow the bacteria the ability of responding to different inducers and inhibitors. On the basis of this mechanism, we could take the E.coli(CL-1) under our command to form different patterns which has mathematically meanings.
How to use it
To form ellipse, we ligate this part with promoter pCons and transform the plasmid into E.coli(CL-1), then we cultivate the bacteria on the plate with M63 medium by controlling the concentration of IPTG and chloromycetin. Doting IPTG on the plate is the most critical procedure on the whole course. By tracking and measuring the chemotaxis diameter of the bacteria on the plate, we get a series of experimental data, More detail in Experimental Data.
To form hyperbolic curve and parabola, we use promoter pBAD which is specifically recognized by L-Arabinose(pTET which is specially recognized by anhydrotetracycline). Then we cultivate the bacteria on the plate with M63 medium controlling the concentrations of IPTG, L-Arabinose (anhydrotetracycline) and chloromycetin. After the M63 medium dries up, we dot the IPTG and L-Arabinose (anhydrotetracycline) on the plate to form the mathematically meaningful pattern. By tracking and measuring the chemotaxis diameter of the bacteria on the plate, we get a series of experimental data, more detail in Experimental Data.
Experimental Data
With K20600(pBAD) as an inhibit promoter
The characterization results of part BBa_K1412001
With R0040(pTET) as an inhibit promoter
The characterization results of part BBa_K1412033
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
More information, click here: [http://2014.igem.org/Team:XMU-China# XMU-China]