Difference between revisions of "Part:BBa K1412002"
 
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='''Basic part of biobrick BBa_K1412001'''=
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='''Composite part of BioBrick [https://parts.igem.org/Part:BBa_K1412001 BBa_K1412001] and [https://parts.igem.org/Part:BBa_K1412033 BBa_K1412033]'''=
  
  
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== What it is ==
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== '''What it is''' ==
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Compared to part BBa_1412001, this part substitutes BBa_R0040, a constant expression promoter in our engineered bacteria CL-1, for BBa_K206000.
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Composite part of BioBrick [https://parts.igem.org/Part:BBa_K1412001 BBa_K1412001] and [https://parts.igem.org/Part:BBa_K1412033 BBa_K1412033] with promoter removed.
  
  
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== What it does ==
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== '''What it does''' ==
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As a control group for K1412001, we use this part to eliminate background expression of AraC, and it’s also capable to form ellipse on the plate under the induction of IPTG.
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Ligating this part with different promoters such as pBAD, pTET, pCons and transform the plasmid into ''E.coli''(''CL-1''), we can endow the bacteria the ability of responding to different inducers and inhibitors. On the basis of this mechanism, we could take the ''E.coli''(''CL-1'') under our command to form different patterns which has mathematically meanings.
  
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== '''How to use it''' ==
  
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== How to use it ==
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To form ellipse, we ligate this part with promoter pCons and transform the plasmid into ''E.coli''(''CL-1''), then we cultivate the bacteria on the plate with M63 medium by controlling the concentration of IPTG and chloromycetin. Doting IPTG on the plate is the most critical procedure on the whole course. By tracking and measuring the chemotaxis diameter of the bacteria on the plate, we get a series of experimental data, More detail in Experimental Data.
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Transforming this part into CL-1, we got the engineering bacteria. Incubate them on the plate with fit concentration of IPTG and chloromycetin, we observed the chemotaxis diameter over time, and got the experimental data. It is also used to form ellipse.
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To form hyperbolic curve and  parabola, we use promoter pBAD which is specifically recognized by L-Arabinose(pTET which is specially recognized by anhydrotetracycline). Then we cultivate the bacteria on the plate with M63 medium  controlling the concentrations of IPTG, L-Arabinose (anhydrotetracycline) and chloromycetin. After the M63 medium dries up, we dot the IPTG and L-Arabinose (anhydrotetracycline) on the plate to form the mathematically meaningful pattern. By tracking and measuring the chemotaxis diameter of the bacteria on the plate, we get a series of experimental data, more detail in Experimental Data.
  
  
 
== '''Experimental Data''' ==
 
== '''Experimental Data''' ==
  
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===With K20600(pBAD) as an inhibit promoter===
  
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[https://parts.igem.org/Part:BBa_K1412001 The characterization results of part BBa_K1412001]
  
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===With R0040(pTET) as an inhibit promoter===
  
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== '''protocol''' ==
 
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1.Transformation
 
  
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2.Extract plasmids
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[https://parts.igem.org/Part:BBa_K1412033 The characterization results of part BBa_K1412033]
  
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3.Digestion
 
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4.DNA gel electrophoresis
 
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5.Gel Extraction
 
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6.Ligation
 
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7.Transformation
 
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8.Extract plasmids
 
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9.Digestion
 
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10.DNA gel electrophoresis
 
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11.Transform the plasmid into CL-1
 
 
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===Usage and Biology===
 
===Usage and Biology===
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== Sequence and Features ==
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== '''Sequence and Features''' ==
 
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</span>
 
<partinfo>BBa_K1412002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1412002 SequenceAndFeatures</partinfo>
  
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----
  
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<I><B>More information, click here: [http://2014.igem.org/Team:XMU-China# XMU-China]
 
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<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K1412002 parameters</partinfo>
 
<partinfo>BBa_K1412002 parameters</partinfo>
 
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Latest revision as of 17:57, 14 October 2014

Composite part of BioBrick BBa_K1412001 and BBa_K1412033

What it is

Composite part of BioBrick BBa_K1412001 and BBa_K1412033 with promoter removed.


What it does

Ligating this part with different promoters such as pBAD, pTET, pCons and transform the plasmid into E.coli(CL-1), we can endow the bacteria the ability of responding to different inducers and inhibitors. On the basis of this mechanism, we could take the E.coli(CL-1) under our command to form different patterns which has mathematically meanings.

How to use it

To form ellipse, we ligate this part with promoter pCons and transform the plasmid into E.coli(CL-1), then we cultivate the bacteria on the plate with M63 medium by controlling the concentration of IPTG and chloromycetin. Doting IPTG on the plate is the most critical procedure on the whole course. By tracking and measuring the chemotaxis diameter of the bacteria on the plate, we get a series of experimental data, More detail in Experimental Data.

To form hyperbolic curve and parabola, we use promoter pBAD which is specifically recognized by L-Arabinose(pTET which is specially recognized by anhydrotetracycline). Then we cultivate the bacteria on the plate with M63 medium controlling the concentrations of IPTG, L-Arabinose (anhydrotetracycline) and chloromycetin. After the M63 medium dries up, we dot the IPTG and L-Arabinose (anhydrotetracycline) on the plate to form the mathematically meaningful pattern. By tracking and measuring the chemotaxis diameter of the bacteria on the plate, we get a series of experimental data, more detail in Experimental Data.


Experimental Data

With K20600(pBAD) as an inhibit promoter

The characterization results of part BBa_K1412001

With R0040(pTET) as an inhibit promoter

The characterization results of part BBa_K1412033

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

More information, click here: [http://2014.igem.org/Team:XMU-China# XMU-China]