Difference between revisions of "Part:BBa K1354001:Design"

(→‎Design Notes)
 
(One intermediate revision by one other user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1354001 short</partinfo>
 
<partinfo>BBa_K1354001 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Enter any design considerations you had to deal with during the detailed design of the sequence.
+
 
 +
The primers for each step were designed and tested.
  
 
===Source===
 
===Source===
  
−
Hi
+
TRP1 was contained in pFA6a.  Using the protocol primer, the knockout cassette was amplified then used in the transformation of yeast to knock out VPS75.  This BioBrick part was taken from the transformed yeast with the VPS75 gene knocked out.
  
 
===References===
 
===References===

Latest revision as of 00:02, 10 October 2014

VPS75 gene deletion cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 339
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 102
    Illegal BamHI site found at 75
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 339
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 339
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The primers for each step were designed and tested.

Source

TRP1 was contained in pFA6a. Using the protocol primer, the knockout cassette was amplified then used in the transformation of yeast to knock out VPS75. This BioBrick part was taken from the transformed yeast with the VPS75 gene knocked out.

References